• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recombinant Antibody, PBS Only

PD-1/CD279 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

EH12.2H7

Cat no : 65586-1-PBS

Print datasheet

Synonyms

CD279, PD1, PD-1, PD 1, hPD-1

验证数据展示

  • FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. This data was developed using the same antibody clone with 65586-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    FC experiment of human PBMCs using 65586-1-MR (same clone as 65586-1-PBS)

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). 1x10^6 PHA treated human PBMCs were surface stained with Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 65586-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.

产品信息

65586-1-PBS targets PD-1/CD279 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, PD 1, hPD-1
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).