验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, THP-1 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12383-1-AP targets PD-ECGF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | PD-ECGF fusion protein Ag3052 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thymidine phosphorylase |
| 别名 | ECGF1, Gliostatin, hPD ECGF, MNGIE, PD ECGF, PDECGF, PD-ECGF, TdRPase, thymidine phosphorylase, TP, TYMP |
| 计算分子量 | 482 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC018160 |
| 基因名称 | Thymidine phosphorylase |
| Gene ID (NCBI) | 1890 |
| RRID | AB_2288047 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19971 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PD-ECGF antibody 12383-1-AP | Download protocol |
| IHC protocol for PD-ECGF antibody 12383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene The proteomic characterization of the peritumor microenvironment in human hepatocellular carcinoma. | ||
Acta Pharmacol Sin Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies. | ||
J Ovarian Res 4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression. | ||
Mol Imaging Biol Evaluation of [(18)F]Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer. | ||
Antioxidants (Basel) D-Penicillamine Reveals the Amelioration of Seizure-Induced Neuronal Injury via Inhibiting Aqp11-Dependent Ferroptosis |
