PD-ECGF Polyclonal antibody
PD-ECGF Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, THP-1 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TYMP Ready-To-Use IHC Kit. TYMP primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12383-1-AP targets PD-ECGF in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | PD-ECGF fusion protein Ag3052 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | thymidine phosphorylase |
| Synonyms | ECGF1, Gliostatin, hPD ECGF, MNGIE, PD ECGF, PDECGF, PD-ECGF, TdRPase, thymidine phosphorylase, TP, TYMP |
| Calculated Molecular Weight | 482 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC018160 |
| Gene Symbol | Thymidine phosphorylase |
| Gene ID (NCBI) | 1890 |
| RRID | AB_2288047 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19971 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PD-ECGF antibody 12383-1-AP | Download protocol |
| IHC protocol for PD-ECGF antibody 12383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene The proteomic characterization of the peritumor microenvironment in human hepatocellular carcinoma. | ||
Acta Pharmacol Sin Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies. | ||
J Ovarian Res 4-Methylumbelliferone inhibits ovarian cancer growth by suppressing thymidine phosphorylase expression. | ||
Mol Imaging Biol Evaluation of [(18)F]Fluorothymidine as a Biomarker for Early Therapy Response in a Mouse Model of Colorectal Cancer. | ||
Antioxidants (Basel) D-Penicillamine Reveals the Amelioration of Seizure-Induced Neuronal Injury via Inhibiting Aqp11-Dependent Ferroptosis |
