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Mouse PD-L1/B7-H1 Recombinant antibody, PBS Only

PD-L1/B7-H1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240993C9

Cat no : 83906-6-PBS

Print datasheet

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

验证数据展示

  • WB analysis of mouse heart using 83906-6-RR (same clone as 83906-6-PBS)

    WB analysis of mouse heart using 83906-6-RR (same clone as 83906-6-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

    IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

    IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83906-6-RR

    Affinity and Kinetic Characterization of 83906-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83906-6-RR against Mouse PD-L1/B7-H1 were performed. The affinity constant is below 1 pM.

产品信息

The immunogen of 83906-6-PBS is PD-L1/B7-H1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD274 antigen
Synonyms Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1
Calculated Molecular Weight33 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_021893.3
Gene Symbol PD-L1
Gene ID (NCBI) 60533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9EP73
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.