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验证数据展示

  • FC experiment of human PBMCs using 98062-1-RR (same clone as 98062-1-PBS)

    FC experiment of human PBMCs using 98062-1-RR (same clone as 98062-1-PBS)

    1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98062-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98062-1-RR (same clone as 98062-1-PBS)

    FC experiment of human PBMCs using 98062-1-RR (same clone as 98062-1-PBS)

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed. This data was developed using the same antibody clone with 98062-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98062-1-RR

    Affinity and Kinetic Characterization of 98062-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98062-1-RR against Human PD-L1/CD274 were performed. The affinity constant is 0.558 nM.

Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody, PBS Only

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98062-1-PBS

产品说明书

CloneNo. 240721H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD274, PD-L1, PD L1, hPD-L1, B7-H1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98062-1-PBS targets PD-L1/CD274 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD274 molecule
别名 CD274, PD-L1, PD L1, hPD-L1, B7-H1
计算分子量 290 aa, 33 kDa
GenBank蛋白编号BC074984
基因名称 PD-L1
Gene ID (NCBI) 29126
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NZQ7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.