验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, PC-3 cells, Raji cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10294-2-AP targets CCM3/PDCD10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CCM3/PDCD10 fusion protein Ag0348 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | programmed cell death 10 |
| 别名 | CCM3, CCM3/PDCD10, PDCD10, programmed cell death 10, TFAR15 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC002506 |
| 基因名称 | PDCD10 |
| Gene ID (NCBI) | 11235 |
| RRID | AB_2162153 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BUL8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCM3/PDCD10 antibody 10294-2-AP | Download protocol |
| IHC protocol for CCM3/PDCD10 antibody 10294-2-AP | Download protocol |
| IP protocol for CCM3/PDCD10 antibody 10294-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol CCM3 is a gatekeeper in focal adhesions regulating mechanotransduction and YAP/TAZ signalling.
| ||
J Clin Invest Mutations in 2 distinct genetic pathways result in cerebral cavernous malformations in mice.
| ||
Development Ccm3, a gene associated with cerebral cavernous malformations, is required for neuronal migration.
| ||
Aging (Albany NY) PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling
| ||
J Cell Sci CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation.
| ||
Hum Mutat A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion . |
