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验证数据展示

  • WB analysis of U-251 using 66440-1-Ig

    WB analysis of U-251 using 66440-1-Ig

    WB result of CCM3/PDCD10 antibody (66440-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDCD10 transfected U-251 cells.

  • WB analysis of Jurkat using 66440-1-Ig

    WB analysis of Jurkat using 66440-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66440-1-Ig

    IHC staining of human colon cancer using 66440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCM3/PDCD10 antibody included
  • IHC staining of human colon cancer using 66440-1-Ig

    IHC staining of human colon cancer using 66440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CCM3/PDCD10 antibody included
  • Featured Product
  • KD/KO验证

CCM3/PDCD10 Monoclonal antibody

CCM3/PDCD10 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66440-1-Ig

产品说明书

CloneNo. 1E5A8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

CCM3, PDCD10, programmed cell death 10, TFAR15

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, human peripheral blood platelets, Ramos cells, U-251 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CCM3/PDCD10 Ready-To-Use IHC Kit. CCM3/PDCD10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66440-1-Ig targets CCM3/PDCD10 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CCM3/PDCD10 fusion protein Ag0348 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 10
别名 CCM3, PDCD10, programmed cell death 10, TFAR15
计算分子量 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC002506
基因名称 PDCD10
Gene ID (NCBI) 11235
RRIDAB_2881810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BUL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development.

实验方案

Product Specific Protocols
WB protocol for CCM3/PDCD10 antibody 66440-1-IgDownload protocol
IHC protocol for CCM3/PDCD10 antibody 66440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols