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PDCD2L Monoclonal antibody

PDCD2L Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G12C4

Cat no : 68249-1-Ig

Print datasheet

Synonyms

PDCD2L, programmed cell death 2 like

验证数据展示

  • WB analysis using 68249-1-Ig

    WB analysis using 68249-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68249-1-Ig

    WB analysis using 68249-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 68249-1-Ig

    IF Staining of HEK-293 using 68249-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDCD2L antibody (68249-1-Ig, Clone: 2G12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HCT 116 cells, HeLa cells, K-562 cells, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68249-1-Ig targets PDCD2L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PDCD2L fusion protein Ag30807 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name programmed cell death 2-like
Synonyms PDCD2L, programmed cell death 2 like
Calculated Molecular Weight 358 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC006146
Gene Symbol PDCD2L
Gene ID (NCBI) 84306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDCD2L was aberrantly expressed in multiple types of human cancers, and associated with clinical stage and molecular subtype and PDCD2L could be served as a biomarker of CRC (PMID: 35216602).

实验方案

Product Specific Protocols
WB protocol for PDCD2L antibody 68249-1-IgDownload protocol
IF protocol for PDCD2L antibody 68249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols