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验证数据展示

  • WB analysis using 12587-1-AP (same clone as 12587-1-PBS)

    WB analysis using 12587-1-AP (same clone as 12587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK293 cells using 12587-1-AP (same clone as 12587-1-PBS)

    WB analysis of HEK293 cells using 12587-1-AP (same clone as 12587-1-PBS)

    WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12587-1-AP (same clone as 12587-1-PBS)

    IHC staining of human breast cancer using 12587-1-AP (same clone as 12587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDCD4 antibody included
  • IP experiment of HeLa using 12587-1-AP (same clone as 12587-1-PBS)

    IP experiment of HeLa using 12587-1-AP (same clone as 12587-1-PBS)

    IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

    FC experiment of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PDCD4 Polyclonal antibody, PBS Only

PDCD4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 12587-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PDCD4 Ready-To-Use IHC Kit. PDCD4 primary antibody included.

产品信息

12587-1-PBS targets PDCD4 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PDCD4 fusion protein Ag3288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 programmed cell death 4 (neoplastic transformation inhibitor)
别名
计算分子量 469 aa, 52 kDa
观测分子量 54-64 kDa
GenBank蛋白编号BC026104
基因名称 PDCD4
Gene ID (NCBI) 27250
RRIDAB_2162296
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53EL6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.