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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12587-1-AP (same clone as 12587-1-PBS)

    WB analysis using 12587-1-AP (same clone as 12587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK293 cells using 12587-1-AP (same clone as 12587-1-PBS)

    WB analysis of HEK293 cells using 12587-1-AP (same clone as 12587-1-PBS)

    WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12587-1-AP (same clone as 12587-1-PBS)

    IHC staining of human breast cancer using 12587-1-AP (same clone as 12587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDCD4 antibody included
  • IP experiment of HeLa using 12587-1-AP (same clone as 12587-1-PBS)

    IP experiment of HeLa using 12587-1-AP (same clone as 12587-1-PBS)

    IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

    FC experiment of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDCD4 Polyclonal antibody, PBS Only

PDCD4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 12587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PDCD4 Ready-To-Use IHC Kit. PDCD4 primary antibody included.

产品信息

12587-1-PBS targets PDCD4 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PDCD4 fusion protein Ag3288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name programmed cell death 4 (neoplastic transformation inhibitor)
Synonyms
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 54-64 kDa
GenBank Accession NumberBC026104
Gene Symbol PDCD4
Gene ID (NCBI) 27250
RRIDAB_2162296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53EL6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.