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  • Featured Product
  • KD/KO Validated

PDCD4 Monoclonal antibody, PBS Only

PDCD4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F1A11

Cat no : 66100-1-PBS

Print datasheet

Synonyms

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

验证数据展示

  • WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

    WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

    WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

    WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

产品信息

66100-1-PBS targets PDCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen PDCD4 fusion protein Ag19239 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name programmed cell death 4 (neoplastic transformation inhibitor)
Synonyms H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC026104
Gene Symbol PDCD4
Gene ID (NCBI) 27250
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53EL6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.