验证数据展示
经过测试的应用
Positive WB detected in | BxPC-3 cells, HeLa cells, HUVEC cells, MCF-7 cells, HEK-293 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84162-3-RR targets PDCD4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | PDCD4 fusion protein Ag3288 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | programmed cell death 4 (neoplastic transformation inhibitor) |
Synonyms | Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug |
Calculated Molecular Weight | 469 aa, 52 kDa |
Observed Molecular Weight | 54-64 kDa |
GenBank Accession Number | BC026104 |
Gene Symbol | PDCD4 |
Gene ID (NCBI) | 27250 |
RRID | AB_3671724 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q53EL6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for PDCD4 antibody 84162-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |