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PDCL Polyclonal antibody

PDCL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16057-1-AP

Print datasheet

Synonyms

PDCL, PhLP, phosducin like, Phosducin like protein

验证数据展示

  • WB analysis using 16057-1-AP

    WB analysis using 16057-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 16057-1-AP

    IHC staining of human placenta using 16057-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 16057-1-AP

    IHC staining of human placenta using 16057-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse brain using 16057-1-AP

    IHC staining of mouse brain using 16057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16057-1-AP (PDCL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, NCI-H1299 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16057-1-AP targets PDCL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PDCL fusion protein Ag9029 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosducin-like
Synonyms PDCL, PhLP, phosducin like, Phosducin like protein
Calculated Molecular Weight 301 aa, 34 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC017227
Gene Symbol PDCL
Gene ID (NCBI) 5082
RRIDAB_2236943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDCL antibody 16057-1-APDownload protocol
IHC protocol for PDCL antibody 16057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Acta Physiol (Oxf)

ATP6V1A is required for synaptic rearrangements and plasticity in murine hippocampal neurons

Authors - Alessandro Esposito
View Article