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PDCL2 Polyclonal antibody

PDCL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17407-1-AP

Print datasheet

Synonyms

GCPHLP, PDCL2, phosducin like 2, Phosducin like protein 2

验证数据展示

  • WB analysis using 17407-1-AP

    WB analysis using 17407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 17407-1-AP

    WB analysis of mouse testis using 17407-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 17407-1-AP

    IHC staining of human kidney using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 17407-1-AP

    IHC staining of human kidney using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17407-1-AP targets PDCL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PDCL2 fusion protein Ag11427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosducin-like 2
Synonyms GCPHLP, PDCL2, phosducin like 2, Phosducin like protein 2
Calculated Molecular Weight 250 aa, 29 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC034431
Gene Symbol PDCL2
Gene ID (NCBI) 132954
RRIDAB_2299492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4E4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDCL2 antibody 17407-1-APDownload protocol
IHC protocol for PDCL2 antibody 17407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Andrology

PDCL2 is essential for sperm acrosome formation and male fertility in mice

Authors - Yoshitaka Fujihara
View Article
humanIHC

Transl Androl Urol

Identification of PDCL2 as a candidate marker in Sertoli cell-only syndrome by chromatin immunoprecipitation-sequencing and bioinformatics analysis

Authors - Yu Yang
View Article
mouseWB

PNAS Nexus

Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida

Authors - Yoshitaka Fujihara
View Article