验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
12442-2-AP targets PDE1A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PDE1A fusion protein Ag3157 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 1A, calmodulin-dependent |
| 别名 | 61 kDa Cam PDE, Cam PDE 1A, hCam 1, HCAM1, HSPDE1A, PDE1A |
| 计算分子量 | 545 aa, 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC022480 |
| 基因名称 | PDE1A |
| Gene ID (NCBI) | 5136 |
| RRID | AB_2162893 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54750 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE1A(Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin(PMID:12135876).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE1A antibody 12442-2-AP | Download protocol |
| IHC protocol for PDE1A antibody 12442-2-AP | Download protocol |
| IP protocol for PDE1A antibody 12442-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biol Reprod Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis. | ||
Biol Reprod Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles†. | ||
Cell Rep Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development. | ||
Front Genet Transcriptome profiling of intact bowel wall reveals that PDE1A and SEMA3D are possible markers with roles in enteric smooth muscle apoptosis, proliferative disorders, and dysautonomia in Crohn's disease |
