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验证数据展示

  • WB analysis using 13121-1-AP

    WB analysis using 13121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13121-1-AP

    WB analysis using 13121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PDE1B Polyclonal antibody

PDE1B Polyclonal Antibody for WB, ELISA
Cat No. 13121-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

63 kDa Cam PDE, Cam PDE 1B, PDE1B, PDE1B1, PDES1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13121-1-AP targets PDE1B in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 PDE1B fusion protein Ag3817 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphodiesterase 1B, calmodulin-dependent
别名 63 kDa Cam PDE, Cam PDE 1B, PDE1B, PDE1B1, PDES1B
计算分子量 536 aa, 61 kDa
观测分子量61 kDa
GenBank蛋白编号BC032226
基因名称 PDE1B
Gene ID (NCBI) 5153
RRIDAB_3085407
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01064
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE1B, also known as PDES1B, belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. PDE1B degrades cAMP and cGMP and regulates various important physiological processes such as proliferation and lipogenesis (PMID: 21960012). PDE1B is enriched in brain tissue. PDE1B gene consists of two isoforms, PDE1B1 (encoding 61 kDa protein with 536 amino acid residues) and PDE1B2 (encoding 59 kDa protein containing 516 amino acid residues).

实验方案

Product Specific Protocols
WB protocol for PDE1B antibody 13121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols