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PDE1B Polyclonal antibody

PDE1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13121-1-AP

Print datasheet

Synonyms

63 kDa Cam PDE, Cam PDE 1B, PDE1B, PDE1B1, PDES1B

验证数据展示

  • WB analysis using 13121-1-AP

    WB analysis using 13121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13121-1-AP

    WB analysis using 13121-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13121-1-AP (PDE1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, mouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13121-1-AP targets PDE1B in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen PDE1B fusion protein Ag3817 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphodiesterase 1B, calmodulin-dependent
Synonyms 63 kDa Cam PDE, Cam PDE 1B, PDE1B, PDE1B1, PDES1B
Calculated Molecular Weight 536 aa, 61 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC032226
Gene Symbol PDE1B
Gene ID (NCBI) 5153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01064
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE1B, also known as PDES1B, belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. PDE1B degrades cAMP and cGMP and regulates various important physiological processes such as proliferation and lipogenesis (PMID: 21960012). PDE1B is enriched in brain tissue. PDE1B gene consists of two isoforms, PDE1B1 (encoding 61 kDa protein with 536 amino acid residues) and PDE1B2 (encoding 59 kDa protein containing 516 amino acid residues).

实验方案

Product Specific Protocols
WB protocol for PDE1B antibody 13121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols