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- KD/KO Validated
PDE2A Polyclonal antibody
PDE2A Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55306-1-AP targets PDE2A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphodiesterase 2A, cGMP-stimulated |
| Synonyms | CGS PDE, cGSPDE, PDE2A, PDE2A1, PED2A4 |
| Calculated Molecular Weight | 106 kDa |
| Observed Molecular Weight | 100-106 kDa |
| GenBank Accession Number | NM_002599 |
| Gene Symbol | PDE2A |
| Gene ID (NCBI) | 5138 |
| RRID | AB_11182279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00408 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE2A antibody 55306-1-AP | Download protocol |
| IHC protocol for PDE2A antibody 55306-1-AP | Download protocol |
| IP protocol for PDE2A antibody 55306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease. | ||
Br J Pharmacol 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner. | ||
Biochem Pharmacol Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A. | ||
Front Pharmacol Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress. | ||
Commun Biol Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy.
| ||
JCI Insight Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity. |
