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PDE3B Polyclonal antibody

PDE3B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25290-1-AP

Print datasheet

Synonyms

EC:3.1.4.17, Cyclic GMP-inhibited phosphodiesterase B, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, cGIPDE1, CGI-PDE B

验证数据展示

  • WB analysis of HL-60 using 25290-1-AP

    WB analysis of HL-60 using 25290-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25290-1-AP

    IP experiment of HeLa using 25290-1-AP

    IP result of anti-PDE3B (IP:25290-1-AP, 5ug; Detection:25290-1-AP 1:300) with HeLa cells lysate 800ug.

经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25290-1-AP targets PDE3B in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PDE3B fusion protein Ag18064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphodiesterase 3B, cGMP-inhibited
Synonyms EC:3.1.4.17, Cyclic GMP-inhibited phosphodiesterase B, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, cGIPDE1, CGI-PDE B
Calculated Molecular Weight 1112 aa, 124 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC136565
Gene Symbol PDE3B
Gene ID (NCBI) 5140
RRIDAB_2880010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13370
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDE3B antibody 25290-1-APDownload protocol
IP protocol for PDE3B antibody 25290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Int J Mol Sci

Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions

Authors - Ning Liu
View Article