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验证数据展示

  • WB analysis using 28573-1-AP

    WB analysis using 28573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28573-1-AP (PDE6D antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28573-1-AP

    WB analysis of HeLa using 28573-1-AP

    WB result of PDE6D antibody (28573-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE6D transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

PDE6D Polyclonal antibody

PDE6D Polyclonal Antibody for WB, ELISA
Cat No. 28573-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PDED

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28573-1-AP targets PDE6D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PDE6D fusion protein Ag29563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphodiesterase 6D, cGMP-specific, rod, delta
Synonyms PDED
Calculated Molecular Weight 17 KDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberNM_002601
Gene Symbol PDE6D
Gene ID (NCBI) 5147
RRIDAB_2881173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43924
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

实验方案

Product Specific Protocols
WB protocol for PDE6D antibody 28573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols