验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17771-1-AP targets PDE7B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | PDE7B fusion protein Ag12184 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 7B |
| 别名 | 3',5'-cyclic-AMP phosphodiesterase 7B, cAMP-specific phosphodiesterase 7B, EC:3.1.4.53, phosphodiesterase 7B |
| 计算分子量 | 450 aa, 52 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC075082 |
| 基因名称 | PDE7B |
| Gene ID (NCBI) | 27115 |
| RRID | AB_2268032 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NP56 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE7B(cAMP-specific 3',5'-cyclic phosphodiesterase 7B) belongs to the cyclic nucleotide phosphodiesterase family and PDE7 subfamily,which regulate intracellular levels of cAMP and cGMP, cyclic nucleotides that play important roles as second messengers in multiple physiologic processes. PDE7B gene is expressed predominantly in the brain and in a number of other tissues including liver, heart, thyroid glands, and skeletal muscle, but not expressed in blood leukocytes (PMID:12388353).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE7B antibody 17771-1-AP | Download protocol |
| IHC protocol for PDE7B antibody 17771-1-AP | Download protocol |
| IF protocol for PDE7B antibody 17771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurobiol Aging Silencing phosphodiesterase 7B gene by lentiviral-shRNA interference attenuates neurodegeneration and motor deficits in hemiparkinsonian mice.
| ||
Stem Cells Phosphodiesterase7 Inhibition Activates Adult Neurogenesis in Hippocampus and Subventricular Zone in vitro and in vivo. | ||
Stem Cells Transl Med Phosphodiesterase 7 inhibition induces dopaminergic neurogenesis in hemiparkinsonian rats. | ||
BMC Cancer Methylation-regulated tumor suppressor gene PDE7B promotes HCC invasion and metastasis through the PI3K/AKT signaling pathway
| ||
Front Immunol The mechanism of PDE7B inhibiting the development of hepatocellular carcinoma through oxidative stress
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