验证数据展示
经过测试的应用
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12648-1-AP targets PDE9A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | PDE9A fusion protein Ag3372 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphodiesterase 9A |
| 别名 | HSPDE9A2, High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A, EC:3.1.4.35 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC009047 |
| 基因名称 | PDE9A |
| Gene ID (NCBI) | 5152 |
| RRID | AB_2161890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O76083 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclic nucleotide phosphodiesterases (PDEs), which hydrolyze the intracellular second messengers cAMP and cGMP to their corresponding monophosphates, play an important role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE9A plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. This protein has 16 isoforms with the MW between 44 kDa and 65 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE9A antibody 12648-1-AP | Download protocol |
| IF protocol for PDE9A antibody 12648-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
