验证数据展示
经过测试的应用
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12648-1-AP targets PDE9A in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | PDE9A fusion protein Ag3372 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphodiesterase 9A |
| Synonyms | HSPDE9A2, High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A, EC:3.1.4.35 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC009047 |
| Gene Symbol | PDE9A |
| Gene ID (NCBI) | 5152 |
| RRID | AB_2161890 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O76083 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclic nucleotide phosphodiesterases (PDEs), which hydrolyze the intracellular second messengers cAMP and cGMP to their corresponding monophosphates, play an important role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE9A plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. This protein has 16 isoforms with the MW between 44 kDa and 65 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDE9A antibody 12648-1-AP | Download protocol |
| IF protocol for PDE9A antibody 12648-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
