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PDF Polyclonal antibody

PDF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24842-1-AP

Print datasheet

Synonyms

PDF, PDF1A, Polypeptide deformylase

验证数据展示

  • WB analysis of MCF-7 using 24842-1-AP

    WB analysis of MCF-7 using 24842-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24842-1-AP

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 24842-1-AP

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24842-1-AP is PDF Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PDF fusion protein Ag18233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptide deformylase (mitochondrial)
Synonyms PDF, PDF1A, Polypeptide deformylase
Calculated Molecular Weight 243 aa, 27 kDa
Observed Molecular Weight 60 kDa
GenBank Accession Numberbc019912
Gene Symbol PDF
Gene ID (NCBI) 64146
RRIDAB_2879753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBH1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDF antibody 24842-1-APDownload protocol
IHC protocol for PDF antibody 24842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols