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PDGFA Polyclonal antibody

PDGFA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26940-1-AP

Print datasheet

Synonyms

PDGF-1, PDGF1, PDGF subunit A, PDGF AA, PDGF A

验证数据展示

  • WB analysis using 26940-1-AP

    WB analysis using 26940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26940-1-AP targets PDGFA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PDGFA fusion protein Ag25643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name platelet-derived growth factor alpha polypeptide
Synonyms PDGF-1, PDGF1, PDGF subunit A, PDGF AA, PDGF A
Calculated Molecular Weight 196 aa, 22 kDa
Observed Molecular Weight13-16 kDa
GenBank Accession NumberBC146594
Gene Symbol PDGFA
Gene ID (NCBI) 5154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDGFA is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFA plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFA is also involved in oligodendrocyte development and the myelination process in the spinal cord and cerebellum. PDGF-A protein monomer is about 13-16 kDa, and PDGF-AA is a 28.5 kDa A chain homodimer.

实验方案

Product Specific Protocols
WB protocol for PDGFA antibody 26940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols