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PDGF-BB Recombinant antibody, PBS Only

PDGF-BB Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230486A7

Cat no : 83214-5-PBS

Print datasheet

Synonyms

PDGFB, PDGFbb, PDGF2, PDGF subunit B, PDGF 2

验证数据展示

  • WB analysis using 83214-5-RR (same clone as 83214-5-PBS)

    WB analysis using 83214-5-RR (same clone as 83214-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83214-5-RR

    Affinity and Kinetic Characterization of 83214-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83214-5-RR against Human PDGFB were performed. The affinity constant is 16.9 nM.

产品信息

83214-5-PBS targets PDGF-BB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PDGF-BB fusion protein Ag25986 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
Synonyms PDGFB, PDGFbb, PDGF2, PDGF subunit B, PDGF 2
Calculated Molecular Weight 241 aa, 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC029822
Gene Symbol PDGFB
Gene ID (NCBI) 5155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01127-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.