• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDGFC Polyclonal antibody

PDGFC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 55076-1-AP

Print datasheet

Synonyms

FALLOTEIN, PDGF C, PDGFC, PDGFC receptor binding form, SCDGF, VEGF E

验证数据展示

  • WB analysis of MDA-MB-453s using 55076-1-AP

    WB analysis of MDA-MB-453s using 55076-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55076-1-AP

    IHC staining of human kidney using 55076-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 55076-1-AP

    IHC staining of human kidney using 55076-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman kidney tissue, human colon tissue, human gliomas tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55076-1-AP targets PDGFC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name platelet derived growth factor C
Synonyms FALLOTEIN, PDGF C, PDGFC, PDGFC receptor binding form, SCDGF, VEGF E
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberNM_016205
Gene Symbol PDGFC
Gene ID (NCBI) 56034
RRIDAB_2881263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRA1
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDGFC is a member of the platelet-derived growth factor family. It is a monomeric protein secreted as a latent dimeric factor. Expression of PDGFC was highest in prostate, testis, and uterus, with weaker expression in spleen, thymus, and small intestine, and little expression in colon and peripheral blood leukocytes.

实验方案

Product Specific Protocols
WB protocol for PDGFC antibody 55076-1-APDownload protocol
IHC protocol for PDGFC antibody 55076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Br J Cancer

Glycometabolic reprogramming-mediated proangiogenic phenotype enhancement of cancer-associated fibroblasts in oral squamous cell carcinoma: role of PGC-1α/PFKFB3 axis.

Authors - Xiang Li
View Article
humanWB

Int J Biol Sci

Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.

Authors - Meicen Zhou
View Article