验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-453s cells |
| Positive IHC detected in | human kidney tissue, human colon tissue, human gliomas tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
55076-1-AP targets PDGFC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | platelet derived growth factor C |
| 别名 | FALLOTEIN, PDGF C, PDGFC, PDGFC receptor binding form, SCDGF, VEGF E |
| 计算分子量 | 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | NM_016205 |
| 基因名称 | PDGFC |
| Gene ID (NCBI) | 56034 |
| RRID | AB_2881263 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDGFC is a member of the platelet-derived growth factor family. It is a monomeric protein secreted as a latent dimeric factor. Expression of PDGFC was highest in prostate, testis, and uterus, with weaker expression in spleen, thymus, and small intestine, and little expression in colon and peripheral blood leukocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFC antibody 55076-1-AP | Download protocol |
| IHC protocol for PDGFC antibody 55076-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Br J Cancer Glycometabolic reprogramming-mediated proangiogenic phenotype enhancement of cancer-associated fibroblasts in oral squamous cell carcinoma: role of PGC-1α/PFKFB3 axis. | ||
Int J Biol Sci Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA. | ||
Oral Dis Fibroblast regulates angiogenesis in assembled oral cancer organoid: A possible role of NNMT |
