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PDGFR alpha/CD140a Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP01256-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01256-4, Human PDGFR alpha/CD140a Recombinant Matched Antibody Pair - PBS only. 84383-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00003. 98141-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP01256-4

    Sandwich ELISA standard curve of MP01256-4, Human PDGFR alpha/CD140a Recombinant Matched Antibody Pair - PBS only. 84383-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00003. 98141-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Serum of six individual healthy human donors was measured. The PDGFR alpha/CD140a concentration of detected samples was determined to be 2,557.7 pg/mL with a range of 1,472.3-4,545.6 pg/mL.

    Sample test of MP01256-4

    Serum of six individual healthy human donors was measured. The PDGFR alpha/CD140a concentration of detected samples was determined to be 2,557.7 pg/mL with a range of 1,472.3-4,545.6 pg/mL.

  • The mean PDGFR alpha/CD140a concentration was determined to be 2.6 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load and 7.0 ng/mL in human placenta cell extract based on a 1.7 mg/mL extract load.

    Sample test of MP01256-4

    The mean PDGFR alpha/CD140a concentration was determined to be 2.6 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load and 7.0 ng/mL in human placenta cell extract based on a 1.7 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01256-4 targets PDGFR alpha/CD140a in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: PDGFR alpha/CD140a Recombinant antibody, PBS Only (Capture) 84383-7-PBS (241658B1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Anti-Human PDGFR alpha/CD140a Rabbit Recombinant Antibody, PBS Only 98141-1-PBS (241658H4). 100 μg. Concentration 1 mgl/ml.

Alternative PDGFR alpha/CD140a matched antibody pairs: MP01256-1, MP01256-2, MP01256-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84383-7-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet-derived growth factor receptor, alpha polypeptide
Synonyms PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor
GenBank accession numberBC015186
Gene symbol PDGFR alpha
UniprotP16234
Gene ID (NCBI) 5156
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 98141-1-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet-derived growth factor receptor, alpha polypeptide
Synonyms PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor
GenBank accession numberBC015186
Gene symbol PDGFR alpha
UniprotP16234
Gene ID (NCBI) 5156
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C