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验证数据展示

  • WB analysis using 84383-2-RR

    WB analysis using 84383-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84383-2-RR (PDGFRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84383-2-RR

    Affinity and Kinetic Characterization of 84383-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84383-2-RR against Human PDGFR alpha/CD140a were performed. The affinity constant is 1.21 nM.

PDGFR alpha/CD140a Recombinant antibody

PDGFR alpha/CD140a Recombinant Antibody for WB, ELISA
Cat No. 84383-2-RR

Print datasheet

CloneNo. 241658G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84383-2-RR targets PDGFR alpha/CD140a in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet-derived growth factor receptor, alpha polypeptide
Synonyms PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor
Calculated Molecular Weight 123 kDa
Observed Molecular Weight170-190 kDa
GenBank Accession NumberBC015186
Gene Symbol PDGFR alpha
Gene ID (NCBI) 5156
RRIDAB_3671919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16234
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

实验方案

Product Specific Protocols
WB protocol for PDGFR alpha/CD140a antibody 84383-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols