验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84383-2-RR targets PDGFR alpha/CD140a in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | platelet-derived growth factor receptor, alpha polypeptide |
| 别名 | PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor |
| 计算分子量 | 123 kDa |
| 观测分子量 | 170-190 kDa |
| GenBank蛋白编号 | BC015186 |
| 基因名称 | PDGFR alpha |
| Gene ID (NCBI) | 5156 |
| RRID | AB_3671919 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16234 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFR alpha/CD140a antibody 84383-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |