验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PDGFRB Ready-To-Use IHC Kit. PDGFRB primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13449-1-AP targets PDGFR beta/CD140b in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PDGFR beta/CD140b fusion protein Ag4285 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | platelet-derived growth factor receptor, beta polypeptide |
| Synonyms | PDGFRB, JTK12, EC:2.7.10.1, CD140B, CD140 antigen-like family member B |
| Calculated Molecular Weight | 1106 aa, 124 kDa |
| Observed Molecular Weight | 160-180 kDa |
| GenBank Accession Number | BC032224 |
| Gene Symbol | PDGFR beta |
| Gene ID (NCBI) | 5159 |
| RRID | AB_2162644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09619 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFR beta/CD140b antibody 13449-1-AP | Download protocol |
| IHC protocol for PDGFR beta/CD140b antibody 13449-1-AP | Download protocol |
| IP protocol for PDGFR beta/CD140b antibody 13449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.
| ||
Cell Rep Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells | ||
J Mol Cell Biol BMP4 mediates the interplay between adipogenesis and angiogenesis during expansion of subcutaneous white adipose tissue. | ||
Br J Cancer Inhibition of PFKFB3 in HER2-positive gastric cancer improves sensitivity to trastuzumab by inducing tumour vessel normalisation. | ||
Neurotherapeutics A switch in the pathway of TRPC3-mediated calcium influx into brain pericytes contributes to capillary spasms after subarachnoid hemorrhage |