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PDGFR beta/CD140b Recombinant antibody

PDGFR beta/CD140b Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230214D11

Cat no : 82943-1-RR

Print datasheet

Synonyms

PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor

验证数据展示

  • WB analysis using 82943-1-RR

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82943-1-RR

    WB analysis using 82943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82943-1-RR (PDGFRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 82943-1-RR

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDGFRB antibody included
  • IHC staining of human placenta using 82943-1-RR

    IHC staining of human placenta using 82943-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82943-1-RR (PDGFR beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDGFRB antibody included
  • Affinity and Kinetic Characterization of 82943-1-RR

    Affinity and Kinetic Characterization of 82943-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82943-1-RR against Human PDGFRB were performed. The affinity constant is 0.668 nM.

经过测试的应用

Positive WB detected inMG-63 cells, HeLa cells, COLO 320 cells, U-87 MG cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDGFRB Ready-To-Use IHC Kit. PDGFRB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82943-1-RR targets PDGFR beta/CD140b in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet-derived growth factor receptor, beta polypeptide
Synonyms PDGFRB, EC:2.7.10.1, CD140B, CD140 antigen-like family member B, Beta-type platelet-derived growth factor receptor
Calculated Molecular Weight 1106 aa, 124 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberBC032224
Gene Symbol PDGFR beta
Gene ID (NCBI) 5159
RRIDAB_3670686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDGFR beta/CD140b antibody 82943-1-RRDownload protocol
IHC protocol for PDGFR beta/CD140b antibody 82943-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols