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PDHA2 Polyclonal antibody

PDHA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17134-1-AP

Print datasheet

Synonyms

PDHA2, PDHAL, PDHE1 A type II

验证数据展示

  • WB analysis using 17134-1-AP

    WB analysis using 17134-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17134-1-AP (PDHA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 17134-1-AP

    IHC staining of mouse testis using 17134-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17134-1-AP (PDHA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDHA2 antibody included

经过测试的应用

Positive WB detected inA431 cells, human placenta tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDHA2 Ready-To-Use IHC Kit. PDHA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17134-1-AP targets PDHA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PDHA2 fusion protein Ag10882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 2
Synonyms PDHA2, PDHAL, PDHE1 A type II
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC030697
Gene Symbol PDHA2
Gene ID (NCBI) 5161
RRIDAB_2878352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29803
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDHA2 antibody 17134-1-APDownload protocol
IHC protocol for PDHA2 antibody 17134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Dis

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Authors - Xinying Cai
View Article