• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDI/P4HB antibody included
  • IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PDI/P4HB antibody included
  • WB analysis of HEK-293 using 66422-1-Ig (same clone as 66422-1-PBS)

    WB analysis of HEK-293 using 66422-1-Ig (same clone as 66422-1-PBS)

    WB result of PDI antibody (66422-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50798-2

    Cytometric bead array standard curve of MP50798-2

    Cytometric bead array standard curve of MP50798-2, PDI Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66422-1-PBS. Detection antibody: 66422-3-PBS. Standard:Ag1747. Range: 0.098-1.563 ng/mL.

  • Featured Product
  • KD/KO验证

PDI Monoclonal antibody, PBS Only (Capture)

PDI Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66422-1-PBS

产品说明书

CloneNo. 2E6A11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PDI/P4HB Ready-To-Use IHC Kit. PDI/P4HB primary antibody included.

产品信息

66422-1-PBS targets PDI as part of a matched antibody pair:

MP50798-2: 66422-1-PBS capture and 66422-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PDI fusion protein Ag1747 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 prolyl 4-hydroxylase, beta polypeptide
别名 P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC014504
基因名称 PDI
Gene ID (NCBI) 5034
RRIDAB_2881794
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07237
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).