• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDILT Polyclonal antibody

PDILT Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26305-1-AP

Print datasheet

Synonyms

PDIA7, PDILT

验证数据展示

  • WB analysis using 26305-1-AP

    WB analysis using 26305-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26305-1-AP (PDILT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours; mouse brain tissue, mouse heart tissue and mouse lung tissue are negative control.

  • IF Staining of mouse testis using 26305-1-AP

    IF Staining of mouse testis using 26305-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 26305-1-AP (PDILT antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human testis using 26305-1-AP

    IHC staining of human testis using 26305-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 26305-1-AP

    IHC staining of human testis using 26305-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26305-1-AP (PDILT Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26305-1-AP targets PDILT in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PDILT fusion protein Ag23675 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein disulfide isomerase-like, testis expressed
Synonyms PDIA7, PDILT
Observed Molecular Weight 67 kDa, 75 kDa
GenBank Accession NumberBC042607
Gene Symbol PDILT
Gene ID (NCBI) 204474
RRIDAB_2880472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDILT, a divergent testis-specific protein disulfide isomerase with a non-classical SXXC motif that engages in disulfide-dependent interactions in the endoplasmic reticulum (PMID: 15475357). PDILT interacts with CALR3 in the testicular germ cells and plays an indispensable role in the disulfide formation in the ADAM3. The PDILT knockout mice were male infertile, consistent with the previous observations that spermatozoa lacking ADAM3 cannot migrate through the UTJ and cannot bind to the ZP (PMID: 22357757). It can be detected as 67 kDa and 75kDa due to the glycosylation.

实验方案

Product Specific Protocols
WB protocol for PDILT antibody 26305-1-APDownload protocol
IHC protocol for PDILT antibody 26305-1-APDownload protocol
IF protocol for PDILT antibody 26305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols