验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse small intestine tissue, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human small intestine tissue, human colon tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 21 publications below |
| IHC | See 3 publications below |
产品信息
10026-1-AP targets PDK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PDK1 fusion protein Ag0025 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyruvate dehydrogenase kinase, isozyme 1 |
| 别名 | PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1 |
| 计算分子量 | 436 aa, 49 kDa |
| 观测分子量 | 46-55 kDa |
| GenBank蛋白编号 | BC039158 |
| 基因名称 | PDK1 |
| Gene ID (NCBI) | 5163 |
| RRID | AB_2161146 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15118 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is speicfic to PDK1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDK1 antibody 10026-1-AP | Download protocol |
| IHC protocol for PDK1 antibody 10026-1-AP | Download protocol |
| IP protocol for PDK1 antibody 10026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect. | ||
Front Endocrinol (Lausanne) HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. | ||
Cells Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression | ||
Front Oncol Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma |
