验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse kidney tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 3 publications below |
产品信息
12215-1-AP targets PDK3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PDK3 fusion protein Ag2879 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyruvate dehydrogenase kinase, isozyme 3 |
| 别名 | PDK3 |
| 计算分子量 | 406 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC015948 |
| 基因名称 | PDK3 |
| Gene ID (NCBI) | 5165 |
| RRID | AB_2877836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15120 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDK3 antibody 12215-1-AP | Download protocol |
| IHC protocol for PDK3 antibody 12215-1-AP | Download protocol |
| IP protocol for PDK3 antibody 12215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma | ||
Mol Cell Biochem Lycorine hydrochloride interferes with energy metabolism to inhibit chemoresistant glioblastoma multiforme cell growth through suppressing PDK3 | ||
Environ Pollut Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse | ||
Nat Commun NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice | ||
Commun Biol Therapeutic potential of microglial SMEK1 in regulating H3K9 lactylation in cerebral ischemia-reperfusion |
