验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse lung tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83583-1-RR targets PDK4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PDK4 fusion protein Ag3629 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | pyruvate dehydrogenase kinase, isozyme 4 |
| 别名 | PDHK4, EC:2.7.11.2, 240551E4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial |
| 计算分子量 | 411 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC040239 |
| 基因名称 | PDK4 |
| Gene ID (NCBI) | 5166 |
| RRID | AB_3671197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16654 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDK4 antibody 83583-1-RR | Download protocol |
| IHC protocol for PDK4 antibody 83583-1-RR | Download protocol |
| FC protocol for PDK4 antibody 83583-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
