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验证数据展示

  • WB analysis of mouse kidney using 83583-1-RR

    WB analysis of mouse kidney using 83583-1-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83583-1-RR (PDK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 83583-1-RR

    IHC staining of mouse skeletal muscle using 83583-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83583-1-RR (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDK4 antibody included
  • FC experiment of HeLa using 83583-1-RR

    FC experiment of HeLa using 83583-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDK4 Recombinant antibody (83583-1-RR, Clone:240551E4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83583-1-RR

    Affinity and Kinetic Characterization of 83583-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83583-1-RR against Human PDK4 were performed. The affinity constant is below 1 pM.

PDK4 Recombinant antibody

PDK4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83583-1-RR

Print datasheet

CloneNo. 240551E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PDHK4, EC:2.7.11.2, 240551E4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse lung tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDK4 Ready-To-Use IHC Kit. PDK4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83583-1-RR targets PDK4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen PDK4 fusion protein Ag3629 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pyruvate dehydrogenase kinase, isozyme 4
Synonyms PDHK4, EC:2.7.11.2, 240551E4, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
Calculated Molecular Weight 411 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC040239
Gene Symbol PDK4
Gene ID (NCBI) 5166
RRIDAB_3671197
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16654
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

实验方案

Product Specific Protocols
WB protocol for PDK4 antibody 83583-1-RRDownload protocol
IHC protocol for PDK4 antibody 83583-1-RRDownload protocol
FC protocol for PDK4 antibody 83583-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols