验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, human lung tissue, human ileum tissue, mouse heart tissue, mouse lung |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11674-1-AP targets PDLIM1/CLP36 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PDLIM1/CLP36 fusion protein Ag2274 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PDZ and LIM domain 1 |
| 别名 | PDLIM1, hCLIM1, Elfin, C-terminal LIM domain protein 1, CLP-36 |
| 计算分子量 | 329 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC000915 |
| 基因名称 | PDLIM1/CLP36 |
| Gene ID (NCBI) | 9124 |
| RRID | AB_2161631 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00151 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| IHC protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| IF protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| IP protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.
| ||
EMBO Rep Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB. | ||
Open Biol Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. | ||
Exp Cell Res CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.
| ||
Vasc Med Decreased PDLIM1 expression in endothelial cells contributes to the development of intracranial aneurysm
|
