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PDLIM3 Polyclonal antibody

PDLIM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13199-2-AP

Print datasheet

Synonyms

PDZ and LIM domain 3, Alpha-actinin-2-associated LIM protein, ALP, Actinin-associated LIM protein, Actinin associated LIM protein

验证数据展示

  • WB analysis of human heart using 13199-2-AP

    WB analysis of human heart using 13199-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 13199-2-AP

    IHC staining of human stomach cancer using 13199-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13199-2-AP (PDLIM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13199-2-AP targets PDLIM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PDLIM3 fusion protein Ag3934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ and LIM domain 3
Synonyms PDZ and LIM domain 3, Alpha-actinin-2-associated LIM protein, ALP, Actinin-associated LIM protein, Actinin associated LIM protein
Calculated Molecular Weight 316 aa, 34 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC027870
Gene Symbol PDLIM3
Gene ID (NCBI) 27295
RRIDAB_2161653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GG5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDLIM3 antibody 13199-2-APDownload protocol
IHC protocol for PDLIM3 antibody 13199-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Small

Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy

Authors - Dan Li
View Article
mouseWB

J Tissue Eng

Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration

Authors - Shijie Fan
View Article