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验证数据展示

  • WB analysis using 18543-1-AP

    WB analysis using 18543-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18543-1-AP (PDLIM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 18543-1-AP

    IHC staining of human skin cancer using 18543-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18543-1-AP (PDLIM4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDLIM4 antibody included
  • IF Staining of U2OS using 18543-1-AP

    IF Staining of U2OS using 18543-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PDLIM4 antibody (18543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PDLIM4 Polyclonal antibody

PDLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18543-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, K-562 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PDLIM4 Ready-To-Use IHC Kit. PDLIM4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18543-1-AP targets PDLIM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PDLIM4 fusion protein Ag13424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ and LIM domain 4
Synonyms RIL, Reversion-induced LIM protein, PDZ and LIM domain 4
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC016765
Gene Symbol PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3085565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50479
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).

实验方案

Product Specific Protocols
WB protocol for PDLIM4 antibody 18543-1-APDownload protocol
IHC protocol for PDLIM4 antibody 18543-1-APDownload protocol
IF protocol for PDLIM4 antibody 18543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols