验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, K-562 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
18543-1-AP targets PDLIM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | PDLIM4 fusion protein Ag13424 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PDZ and LIM domain 4 |
Synonyms | RIL, Reversion-induced LIM protein, PDZ and LIM domain 4 |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC016765 |
Gene Symbol | PDLIM4 |
Gene ID (NCBI) | 8572 |
RRID | AB_3085565 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P50479 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).
实验方案
Product Specific Protocols | |
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WB protocol for PDLIM4 antibody 18543-1-AP | Download protocol |
IHC protocol for PDLIM4 antibody 18543-1-AP | Download protocol |
IF protocol for PDLIM4 antibody 18543-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |