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PDLIM5 Polyclonal antibody
PDLIM5 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, HUVEC cells, NIH/3T3 cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10530-1-AP targets PDLIM5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | PDLIM5 fusion protein Ag0808 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PDZ and LIM domain 5 |
Synonyms | ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 63-68 kDa |
GenBank Accession Number | BC008741 |
Gene Symbol | PDLIM5 |
Gene ID (NCBI) | 10611 |
RRID | AB_2161779 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96HC4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
IHC protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
IP protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Death Differ MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis.
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Oncotarget High expression of PDLIM5 facilitates cell tumorigenesis and migration by maintaining AMPK activation in prostate cancer.
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Drug Deliv Ultrasound-sensitive siRNA-loaded nanobubbles fabrication and antagonism in drug resistance for NSCLC. | ||
J Mol Cell Cardiol Extracellular vesicular MicroRNA-27a* contributes to cardiac hypertrophy in chronic heart failure.
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Chem Biol Drug Des si-RNA mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro.
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Sci Rep Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration |