验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HUVEC cells, NIH/3T3 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10530-1-AP targets PDLIM5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PDLIM5 fusion protein Ag0808 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PDZ and LIM domain 5 |
| 别名 | PDZ and LIM domain 5, L9, Enigma-like PDZ and LIM domains protein, ENH |
| 计算分子量 | 64 kDa |
| 观测分子量 | 63-68 kDa |
| GenBank蛋白编号 | BC008741 |
| 基因名称 | PDLIM5 |
| Gene ID (NCBI) | 10611 |
| RRID | AB_2161779 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96HC4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression. This antibody is specific to PDLIM5, and its specificity has been confirmed by siRNA test.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
| IHC protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
| IP protocol for PDLIM5 antibody 10530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis.
| ||
Oncotarget High expression of PDLIM5 facilitates cell tumorigenesis and migration by maintaining AMPK activation in prostate cancer.
| ||
Drug Deliv Ultrasound-sensitive siRNA-loaded nanobubbles fabrication and antagonism in drug resistance for NSCLC. | ||
J Mol Cell Cardiol Extracellular vesicular MicroRNA-27a* contributes to cardiac hypertrophy in chronic heart failure.
| ||
Chem Biol Drug Des si-RNA mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro.
| ||
Sci Rep Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration |
