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验证数据展示

  • WB analysis of HeLa using 17080-1-AP

    WB analysis of HeLa using 17080-1-AP

    WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

  • WB analysis using 17080-1-AP

    WB analysis using 17080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17080-1-AP

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • IHC staining of human liver using 17080-1-AP

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • IP experiment of HeLa using 17080-1-AP

    IP experiment of HeLa using 17080-1-AP

    IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:5000) with HeLa cells lysate 1200 ug.

  • IHC staining of human colon using 17080-1-AP

    IHC staining of human colon using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDLIM7 antibody included
  • Featured Product
  • KD/KO验证

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17080-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

LMP1, LIM mineralization protein, ENIGMA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human colon, human kidney tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDLIM7 Ready-To-Use IHC Kit. PDLIM7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17080-1-AP targets PDLIM7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 PDLIM7 fusion protein Ag1334 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ and LIM domain 7 (enigma)
别名 LMP1, LIM mineralization protein, ENIGMA
计算分子量 50 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC014521
基因名称 PDLIM7
Gene ID (NCBI) 9260
RRIDAB_2161788
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDLIM7 antibody 17080-1-APDownload protocol
IHC protocol for PDLIM7 antibody 17080-1-APDownload protocol
IP protocol for PDLIM7 antibody 17080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols