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PDP2 Polyclonal antibody

PDP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13404-1-AP

Print datasheet

Synonyms

KIAA1348, PDP 2, PDP2, PDPC 2

验证数据展示

  • WB analysis of mouse skeletal muscle using 13404-1-AP

    WB analysis of mouse skeletal muscle using 13404-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13404-1-AP

    IHC staining of mouse brain using 13404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 13404-1-AP

    IHC staining of mouse brain using 13404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, HeLa cells, mouse kidney tissue
Positive IHC detected inmouse brain tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13404-1-AP targets PDP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PDP2 fusion protein Ag4257 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyruvate dehydrogenase phosphatase isoenzyme 2
Synonyms KIAA1348, PDP 2, PDP2, PDPC 2
Calculated Molecular Weight 529 aa, 60 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC028030
Gene Symbol PDP2
Gene ID (NCBI) 57546
RRIDAB_10639505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDP2, is the Ca2+-insensitive isoform of pyruvate dehydrogenase phosphataseis and found in liver and adipose tissue. In adipose tissue,insulin reduces the concentration dependence of PDP2 activity for Mg2+(PMID:12676647).It catalyzes the dephosphorylation and concomitant reactivation of the mitochondrial pyruvate dehydrogenase multienzyme complex.It can be detected the mature (52 kDa) and the immature (60 kDa) bands in western blot.

实验方案

Product Specific Protocols
WB protocol for PDP2 antibody 13404-1-APDownload protocol
IHC protocol for PDP2 antibody 13404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Dev Cell

Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.

Authors - Wanchun Yang
View Article
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
View Article