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- KD/KO Validated
PDPK1 Polyclonal antibody
PDPK1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, LNCaP cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PDPK1 Ready-To-Use IHC Kit. PDPK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17086-1-AP targets PDPK1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, WB, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | PDPK1 fusion protein Ag9213 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | 3-phosphoinositide dependent protein kinase-1 |
| Synonyms | hPDK1, PDK1, PDPK1, PRO0461 |
| Calculated Molecular Weight | 556 aa, 63 kDa |
| Observed Molecular Weight | 60-63 kDa |
| GenBank Accession Number | BC012103 |
| Gene Symbol | PDPK1 |
| Gene ID (NCBI) | 5170 |
| RRID | AB_2161289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDPK1 antibody 17086-1-AP | Download protocol |
| IHC protocol for PDPK1 antibody 17086-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell A Non-canonical PDK1-RSK Signal Diminishes Pro-caspase-8-Mediated Necroptosis Blockade.
| ||
PLoS Biol Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex. | ||
Free Radic Biol Med Redox sensor NPGPx restrains ZAP70 activity and modulates T cell homeostasis. | ||
Front Immunol Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer
| ||
J Innate Immun Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. | ||
Biol Trace Elem Res HIF-1α Mediates Arsenic-Induced Metabolic Reprogramming in Lung Bronchial Epithelial Cells. |
