- Featured Product
- KD/KO Validated
PDS5A Polyclonal antibody
PDS5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse kidney tissue, COLO 320 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
17485-1-AP targets PDS5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | PDS5A fusion protein Ag11525 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) |
| Synonyms | SCC112, SCC 112, PIG54, PDS5, KIAA0648 |
| Calculated Molecular Weight | 1297 aa, 147 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC009650 |
| Gene Symbol | PDS5A |
| Gene ID (NCBI) | 23244 |
| RRID | AB_2162088 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q29RF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDS5A antibody 17485-1-AP | Download protocol |
| IHC protocol for PDS5A antibody 17485-1-AP | Download protocol |
| IF protocol for PDS5A antibody 17485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin | ||
