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PDS5A Monoclonal antibody

PDS5A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8D4

Cat no : 67520-1-Ig

Print datasheet

Synonyms

SCC 112, PIG54, PDS5, KIAA0648, Cell proliferation-inducing gene 54 protein

验证数据展示

  • WB analysis using 67520-1-Ig

    WB analysis using 67520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67520-1-Ig (PDS5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67520-1-Ig

    IHC staining of human breast cancer using 67520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 67520-1-Ig

    WB analysis of HeLa using 67520-1-Ig

    WB result of PDS5A antibody (67520-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HeLa cells.

  • IF Staining of A431 using 67520-1-Ig

    IF Staining of A431 using 67520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 67520-1-Ig

    IF Staining of HepG2 using 67520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67520-1-Ig targets PDS5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PDS5A fusion protein Ag11525 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
Synonyms SCC 112, PIG54, PDS5, KIAA0648, Cell proliferation-inducing gene 54 protein
Calculated Molecular Weight 1297 aa, 147 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC009650
Gene Symbol PDS5A
Gene ID (NCBI) 23244
RRIDAB_2882740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ29RF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDS5A antibody 67520-1-IgDownload protocol
IHC protocol for PDS5A antibody 67520-1-IgDownload protocol
IF protocol for PDS5A antibody 67520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols