• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDXDC1 Polyclonal antibody

PDXDC1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21021-1-AP

Print datasheet

Synonyms

KIAA0251, LP8165, PDXDC1

验证数据展示

  • WB analysis using 21021-1-AP

    WB analysis using 21021-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 21021-1-AP

    IP experiment of HepG2 using 21021-1-AP

    IP result of anti-PDXDC1 (IP:21021-1-AP, 4ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.

  • IF Staining of HepG2 using 21021-1-AP

    IF Staining of HepG2 using 21021-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human hepatocirrhosis using 21021-1-AP

    IHC staining of human hepatocirrhosis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 21021-1-AP

    IHC staining of human hepatocirrhosis using 21021-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue, HeLa cells, SGC-7901 cells, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21021-1-AP is PDXDC1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PDXDC1 fusion protein Ag15283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyridoxal-dependent decarboxylase domain containing 1
Synonyms KIAA0251, LP8165, PDXDC1
Calculated Molecular Weight 788 aa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC033748
Gene Symbol PDXDC1
Gene ID (NCBI) 23042
RRIDAB_10695768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P996
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PDXDC1 antibody 21021-1-APDownload protocol
IHC protocol for PDXDC1 antibody 21021-1-APDownload protocol
IF protocol for PDXDC1 antibody 21021-1-APDownload protocol
IP protocol for PDXDC1 antibody 21021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Mol Med (Berl)

Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight.

Authors - Yu-Qian Song
View Article
mouseWB

Cell Mol Life Sci

Protease-independent control of parthanatos by HtrA2/Omi

Authors - Jonas Weiß
View Article