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PDXK Polyclonal antibody

PDXK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15309-1-AP

Print datasheet

Synonyms

C21orf124, C21orf97, PDXK, PKH, PNK, PRED79, Pyridoxal kinase, Pyridoxine kinase

验证数据展示

  • WB analysis using 15309-1-AP

    WB analysis using 15309-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15309-1-AP (PDXK antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 15309-1-AP

    IP experiment of mouse liver using 15309-1-AP

    IP result of anti-PDXK (IP:15309-1-AP, 4ug; Detection:15309-1-AP 1:500) with mouse liver tissue lysate 6000 ug.

  • IHC staining of human urothelial carcinoma using 15309-1-AP

    IHC staining of human urothelial carcinoma using 15309-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15309-1-AP (PDXK antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PDXK antibody included

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, mouse liver tissue, mouse spinal cord, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PDXK Ready-To-Use IHC Kit. PDXK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15309-1-AP targets PDXK in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PDXK fusion protein Ag7451 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyridoxal (pyridoxine, vitamin B6) kinase
Synonyms C21orf124, C21orf97, PDXK, PKH, PNK, PRED79, Pyridoxal kinase, Pyridoxine kinase
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 40 kDa, 35 kDa
GenBank Accession NumberBC000123
Gene Symbol PDXK
Gene ID (NCBI) 8566
RRIDAB_2252232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDXK encodes for a pyridoxal kinase, which converts inactive B6 vitamers to the active cofactor pyridoxal 5'-phosphate (PLP).

实验方案

Product Specific Protocols
WB protocol for PDXK antibody 15309-1-APDownload protocol
IHC protocol for PDXK antibody 15309-1-APDownload protocol
IP protocol for PDXK antibody 15309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Natural product P57 induces hypothermia through targeting pyridoxal kinase

Authors - Ruina Wang
View Article
mouseIF,IHC

Cell Rep

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease

Authors - Min Yu
View Article
humanWB

Drug Deliv Transl Res

Delivery of exogenous miR-19b by Wharton's Jelly Mesenchymal Stem Cells attenuates transplanted kidney ischemia/reperfusion injury by regulating cellular metabolism

Authors - Xiaoqiang Wu
View Article