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PDXP Polyclonal antibody

PDXP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27490-1-AP

Print datasheet

Synonyms

CIN, PDXP, PLP, PLP phosphatase, PLPP

验证数据展示

  • WB analysis using 27490-1-AP

    WB analysis using 27490-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27490-1-AP (PDXP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27490-1-AP

    IHC staining of human liver using 27490-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27490-1-AP (PDXP antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27490-1-AP

    IHC staining of human liver using 27490-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27490-1-AP (PDXP antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, Neuro-2a cells, mouse brain tissue
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27490-1-AP targets PDXP in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen PDXP fusion protein Ag26592 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyridoxal (pyridoxine, vitamin B6) phosphatase
Synonyms CIN, PDXP, PLP, PLP phosphatase, PLPP
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC064922
Gene Symbol PDXP
Gene ID (NCBI) 57026
RRIDAB_2880887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDXP, also named CIN, belongs to the HAD-like hydrolase superfamily. As a pyridoxal phosphate (PLP) phosphatase, PDXP can catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP and therefore plays a role in vitamin B6 metabolism. PDXP is highly expressed in all the regions of central nerve system except the spinal cord. PDXP is also expressed at high level in liver and testis.

实验方案

Product Specific Protocols
WB protocol for PDXP antibody 27490-1-APDownload protocol
IHC protocol for PDXP antibody 27490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Mol Carcinog

p110CUX1 promotes acute myeloid leukemia progression via regulating pyridoxal phosphatase expression and activating PI3K/AKT/mTOR signaling pathway

Authors - Hongyan Zhang
  • KD Validated
View Article