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PDZD2 Polyclonal antibody

PDZD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30191-1-AP

Print datasheet

Synonyms

AIPC, KIAA0300, PAPIN, PDZ domain containing 2, PDZD2, PDZK3, PIN1

验证数据展示

  • WB analysis using 30191-1-AP

    WB analysis using 30191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30191-1-AP (PDZD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 30191-1-AP

    IHC staining of mouse lung using 30191-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30191-1-AP (PDZD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 30191-1-AP

    IHC staining of mouse lung using 30191-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30191-1-AP (PDZD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30191-1-AP targets PDZD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PDZD2 fusion protein Ag32900 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ domain containing 2
Synonyms AIPC, KIAA0300, PAPIN, PDZ domain containing 2, PDZD2, PDZK3, PIN1
Calculated Molecular Weight302 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberNM_178140
Gene Symbol PDZD2
Gene ID (NCBI) 23037
RRIDAB_3086258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15018
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDZD2, PDZ-domain containing-2, also named as AIPC, KIAA0300 and PDZK3, is associated with the early promotion of prostate tumoregenesis. The antibody recognizes near the N-term of PDZD2. PDZD2 is a novel factor that affects the growth and differentiation of human fetal pancreatic progenitor cells. The secreted form sPDZD2 can be detected 37 kDa (PMID: 18037333).

实验方案

Product Specific Protocols
WB protocol for PDZD2 antibody 30191-1-APDownload protocol
IHC protocol for PDZD2 antibody 30191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols