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验证数据展示

  • WB analysis using 30191-1-AP

    WB analysis using 30191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30191-1-AP (PDZD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 30191-1-AP

    IHC staining of mouse lung using 30191-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30191-1-AP (PDZD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 30191-1-AP

    IHC staining of mouse lung using 30191-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30191-1-AP (PDZD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDZD2 Polyclonal antibody

PDZD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30191-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

AIPC, KIAA0300, PAPIN, PDZ domain containing 2, PDZD2, PDZK3, PIN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30191-1-AP targets PDZD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 PDZD2 fusion protein Ag32900 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ domain containing 2
别名 AIPC, KIAA0300, PAPIN, PDZ domain containing 2, PDZD2, PDZK3, PIN1
计算分子量302 kDa
观测分子量37 kDa
GenBank蛋白编号NM_178140
基因名称 PDZD2
Gene ID (NCBI) 23037
RRIDAB_3086258
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15018
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDZD2, PDZ-domain containing-2, also named as AIPC, KIAA0300 and PDZK3, is associated with the early promotion of prostate tumoregenesis. The antibody recognizes near the N-term of PDZD2. PDZD2 is a novel factor that affects the growth and differentiation of human fetal pancreatic progenitor cells. The secreted form sPDZD2 can be detected 37 kDa (PMID: 18037333).

实验方案

Product Specific Protocols
WB protocol for PDZD2 antibody 30191-1-APDownload protocol
IHC protocol for PDZD2 antibody 30191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols