验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10507-2-AP targets PDZK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PDZK1 fusion protein Ag0787 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PDZ domain containing 1 |
| 别名 | CAP70, CLAMP, NaPi Cap1, NHERF 3, NHERF3, PDZ domain containing 1, PDZD1, PDZK1 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC006496 |
| 基因名称 | PDZK1 |
| Gene ID (NCBI) | 5174 |
| RRID | AB_2299495 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T2W1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| IP protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Downregulation of vimentin expression increased drug resistance in ovarian cancer cells. | ||
Mol Med PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth. | ||
Mol Med Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37. | ||
Cell Div miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism
| ||
Aging (Albany NY) Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1
| ||
Bone Res PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function |
