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PECR Monoclonal antibody

PECR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D3G1

Cat no : 68248-1-Ig

Print datasheet

Synonyms

DCR RP, HPDHase, HSA250303, PECR, pVI ARL, SDR29C1, TERP

验证数据展示

  • WB analysis using 68248-1-Ig

    WB analysis using 68248-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68248-1-Ig (PECR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68248-1-Ig targets PECR in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PECR fusion protein Ag7022 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxisomal trans-2-enoyl-CoA reductase
Synonyms DCR RP, HPDHase, HSA250303, PECR, pVI ARL, SDR29C1, TERP
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC002529
Gene Symbol PECR
Gene ID (NCBI) 55825
RRIDAB_2935334
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BY49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxisomal trans-2-enoyl-CoA reductase (PECR) is also called TERP and TECR, which is located on chromosome q35 with 86627 bases. It can participate in carbon chain elongation in fatty acid metabolism and catalyze the last reaction of four long chain fatty acid elongation cycles. Each cycle of PECR adds two carbons to the long chain and very long chain fatty acid (VLCFA) chains, reducing the intermediate of trans-2,3-enoyl coenzyme A fatty acid to acyl coenzyme A, which can further extend the carbon chain by entering a new elongation cycle. Meanwhile, PECR is a peroxisome protein involved in fatty acid synthesis and plays an important role in milk fat synthesis. The molecular mass of PECR is 33 kDa. (PMID: 31467878)

实验方案

Product Specific Protocols
WB protocol for PECR antibody 68248-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols