验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, human serum |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
26045-1-AP targets Serpin F1/PEDF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Serpin F1/PEDF fusion protein Ag23296 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 |
| 别名 | PIG35, PEDF, Pigment epithelium-derived factor, EPC-1, EPC 1 |
| 计算分子量 | 418 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC013984 |
| 基因名称 | PEDF |
| Gene ID (NCBI) | 5176 |
| RRID | AB_2880349 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P36955 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Serpin F1/PEDF antibody 26045-1-AP | Download protocol |
| IHC protocol for Serpin F1/PEDF antibody 26045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization | ||
Hum Gene Ther Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor. | ||
Cell Death Dis TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway. |