PEG10 Polyclonal antibody

PEG10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14412-1-AP

Print datasheet

Synonyms

EDR, KIAA1051e, KIAA1051, HB1, HB 1



经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, PC-13 cells, mouse liver tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PEG10 Ready-To-Use IHC Kit. PEG10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14412-1-AP targets PEG10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PEG10 fusion protein Ag5754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paternally expressed 10
Synonyms EDR, KIAA1051e, KIAA1051, HB1, HB 1
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 50 kDa, 100 kDa
GenBank Accession NumberBC050659
Gene Symbol PEG10
Gene ID (NCBI) 23089
RRIDAB_10694427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEG10 antibody 14412-1-APDownload protocol
IHC protocol for PEG10 antibody 14412-1-APDownload protocol
IF protocol for PEG10 antibody 14412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Cell

Cellular architecture of human brain metastases.

Authors - Hugo Gonzalez
humanWB

Acta Pharmacol Sin

Transcriptional regulator CTR9 promotes hepatocellular carcinoma progression and metastasis via increasing PEG10 transcriptional activity.

Authors - Bin Zhang
humanWB,IF

bioRxiv

Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties

Authors - Harihar M Mohan
humanIF

Elife

UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS

Authors - Holly H Black
humanWB

Ann Med

A 12-gene panel in estimating hormone-treatment responses of castration-resistant prostate cancer patients generated using a combined analysis of bulk and single-cell sequencing data

Authors - Juanlan Huang
humanIHC

J Exp Clin Cancer Res

Targeting PEG10 as a novel therapeutic approach to overcome CDK4/6 inhibitor resistance in breast cancer

Authors - Nar Bahadur Katuwal